goat polyclonal anti oct4  (R&D Systems)

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: OCT4/POU5F1 is required for NANOG expression in bovine blastocysts

doi: 10.1073/pnas.1718833115

Figure Lengend Snippet: (A) Experimental procedure to produce OCT4 KO embryos through SCNT. (B) Single guide RNA (sgRNA) design to mutate exon 2 of OCT4; biallelic deletion of single nucleotide in OCT4KOtm1; and maintained SNP. (C) PCA of transcriptome profiles from individual day 7 OCT4KOtm1 (n = 5), NT Ctrl (n = 3), and IVP Ctrl blastocysts (n = 3). PC1, principal component 1; PC2, principal component 2. (D) Venn diagram of differentially abundant transcripts (DATs) identified by DESeq2 analysis of the NT Ctrl vs. IVP Ctrl, OCT4KOtm1 vs. NT Ctrl, and OCT4KOtm1 vs. IVP Ctrl blastocyst transcriptome data. (E) Heat map of DATs from DESeq2 [n = 625; adjusted P value (Padj) < 0.05]. (F) Heat map of genes specific for EPI (blue), HB (red), and TE (green); asterisks indicate DATs at Padj < 0.05.

Article Snippet: For OCT4/CDX2 staining, dilutions of goat anti-human OCT4 polyclonal antibodies (SC8628; Santa Cruz) and rabbit anti-human CDX2 polyclonal antibodies (ab88129; Abcam) were 1:500 and 1:250, respectively.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: OCT4/POU5F1 is required for NANOG expression in bovine blastocysts

doi: 10.1073/pnas.1718833115

Figure Lengend Snippet: Representative confocal planes of day 5 morulae stained for OCT4/CDX2 (Left) and NANOG/GATA6 (Right) from OCT4KOtm1, NT Ctrl, and IVP Ctrl embryos. Sample sizes of OCT4/CDX2 and NANOG/GATA6 were n = 6, 3, and 10 and n = 9, 14, and 7 for OCT4KOtm1, NT Ctrl, and IVP Ctrl, respectively. (Scale bars, 100 µm.)

Article Snippet: For OCT4/CDX2 staining, dilutions of goat anti-human OCT4 polyclonal antibodies (SC8628; Santa Cruz) and rabbit anti-human CDX2 polyclonal antibodies (ab88129; Abcam) were 1:500 and 1:250, respectively.

Techniques: Staining

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: OCT4/POU5F1 is required for NANOG expression in bovine blastocysts

doi: 10.1073/pnas.1718833115

Figure Lengend Snippet: Representative confocal plane of day 7 blastocysts stained against OCT4/CDX2 (Left) and NANOG/GATA6 (Right) from OCT4KOtm1, NT Ctrl, and IVP Ctrl embryos. Sample sizes of OCT4/CDX2 and NANOG/GATA6 were n = 24, 20, and 40 and n = 21, 23, and 9 for OCT4KOtm1, NT Ctrl, and IVP Ctrl, respectively. (Scale bars, 100 µm.)

Article Snippet: For OCT4/CDX2 staining, dilutions of goat anti-human OCT4 polyclonal antibodies (SC8628; Santa Cruz) and rabbit anti-human CDX2 polyclonal antibodies (ab88129; Abcam) were 1:500 and 1:250, respectively.

Techniques: Staining

Journal: Scientific Reports

Article Title: Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue

doi: 10.1038/srep45577

Figure Lengend Snippet: ( a – c ) The number of cells after dinaciclib treatment. Cell number in 49 fields (7 × 7) was calculated and shown in the graph (n = 4). ( a ) Total cell number. **p < 0.01 vs control. ( b ) Number of OCT4 positive cells. **p < 0.01 vs control. ( c ) Number of OCT4 negative cells. n.s., not significant. ( d ) The percentage of OCT4 positive cells. **p < 0.01 vs control. *p < 0.05 vs 6 nM. ( e ) Representative images of OCT4 positive cells (red). Nuclei were stained with Hoechst (blue). Scale bars = 200 μm.

Article Snippet: For immunocytochemistry: mouse monoclonal anti-cardiac troponin T antibody (cTnT; Thermo Scientific, Rockford, IL, USA), goat polyclonal anti-OCT4 antibody (R&D systems, Minneapolis, MN, USA), rabbit polyclonal anti-Ki67 antibody (Abcam, Cambridge, UK).

Techniques: Control, Staining